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dc.date.accessioned 2012-02-06T13:12:43Z
dc.date.available 2012-02-06T03:00:00Z
dc.date.issued 2011 es
dc.identifier.uri http://hdl.handle.net/10915/8420
dc.description.abstract A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of ramosetron in rat plasma using one-step protein precipitation was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographically separation was achieved on an SB-C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1 % formic acid as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 280 for ramosetron and m/z 326 for the IS. Calibration plots were linear over the range of 10-1000 ng/mL for ramosetron in rat plasma. Lower limit of quantification (LLOQ) for Ramosetron was 10 ng/mL. Mean recovery of ramosetron from plasma was in the range of 88.5-92.8 %. CV of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic study for determination of ramosetron in rat plasma. es
dc.format.extent p. 2076-2080 es
dc.language en es
dc.title Determination of ramosetron in rat plasma by LC-ESI-MS and its application es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_2_5.pdf es
sedici.creator.person Lin, Guanyang es
sedici.creator.person Chen, Xiaole es
sedici.creator.person Zheng, Meiqin es
sedici.creator.person Wang, Youpei es
sedici.creator.person Pang, Lingxia es
sedici.creator.person Wang, Qing es
sedici.subject.materias Farmacia es
sedici.subject.decs Plasma es
sedici.subject.decs Cromatografía Liquida es
sedici.subject.decs Espectrometría de Masas es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Comunicacion es
sedici.description.peerReview peer-review es
sedici2003.identifier ARG-FARM-ART-0000001908 es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 30, no. 10 es


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