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dc.date.accessioned 2012-03-27T12:36:12Z
dc.date.available 2012-03-27T03:00:00Z
dc.date.issued 2012 es
dc.identifier.uri http://hdl.handle.net/10915/8421
dc.description.abstract A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of dapoxetine in rat plasma using one-step protein precipitation was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used in sample preparation. Chromatographically separation was achieved on an SB-C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1 % formic acid as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 306 for dapoxetine and m/z 326 for the IS. Calibration plots were linear over the range of 5-1000 ng/mL for dapoxetine in rat plasma. Lower limit of quantification (LLOQ) for dapoxetine was 5 ng/mL. Mean recovery of dapoxetine from plasma was in the range of 92.4-98.6 %. CV of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of dapoxetine in rat plasma. es
dc.format.extent p. 5-9 es
dc.language en es
dc.title Development and validation of LC-ESI-MS method for the quantification of dapoxetine in rat plasma es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_1.pdf es
sedici.creator.person Ma, Jianshe es
sedici.creator.person Luo, Shunbin es
sedici.creator.person Chen, Chan es
sedici.creator.person Wang, Jinjin es
sedici.creator.person Wang, Zhibin es
sedici.creator.person Wang, Zhiyi es
sedici.subject.materias Farmacia es
sedici.subject.decs Serotonina es
sedici.subject.decs Espectrometría de Masas es
sedici.subject.decs Cromatografía Liquida es
sedici.subject.decs Plasma es
sedici.subject.decs Ratas es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Articulo es
sedici.description.peerReview peer-review es
sedici2003.identifier ARG-FARM-ART-0000001909 es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 31, no. 1 es

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