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dc.date.accessioned | 2019-10-28T16:24:24Z | |
dc.date.available | 2019-10-28T16:24:24Z | |
dc.date.issued | 2008 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/84230 | |
dc.description.abstract | Islet neogenesis associated protein (INGAP) increases islet mass and insulin secretion in neonatal and adult rat islets. In the present study, we measured the short- and long-term effects of INGAP-PP (a pentadecapeptide having the 104-118 amino acid sequence of INGAP) upon islet protein expression and phosphorylation of components of the P13K, MAPK and cholinergic pathways, and on insulin secretion. Short-term exposure of neonatal islets to INGAP-PP (90 s, 5, 15, and 30 min) significantly increased Akt1-Ser473 and MAPK3/1-Thr202/ Tyr204 phosphorylation and INGAP-PP also acutely increased insulin secretion from islets perifused with 2 and 20 mM glucose. Islets cultured for 4 days in the presence of INGAP-PP showed an increased expression of Akt1, Frap1, and Mapk1 mRNAs as well as of the muscarinic M3 receptor subtype, and phospholipase C (PLC)-β2 proteins. These islets also showed increased Akt1 and MAPK3/1 protein phosphorylation. Brief exposure of INGAP-PP-treated islets to carbachol (Cch) significantly increased P70S6K-Thr389 and MAPK3/1 phosphorylation and these islets released more insulin when challenged with Cch that was prevented by the M3 receptor antagonist 4-DAMP, in a concentration-dependent manner. In conclusion, these data indicate that short- and long-term exposure to INGAP-PP significantly affects the expression and the phosphorylation of proteins involved in islet P13K and MAPK signaling pathways. The observations of INGAPP-PP-stimulated up-regulation of cholinergic M3 receptors and PLC- proteins, enhanced P70S6K and MAPK3/1 phosphorylation and Cch-induced insulin secretion suggest a participation of the cholinergic pathway in INGAP-PP-mediated effects. | en |
dc.format.extent | 299-306 | es |
dc.language | en | es |
dc.subject | Islet neogenesis associated protein | es |
dc.subject | insulin secretion | es |
dc.title | Islet neogenesis-associated protein signaling in neonatal pancreatic rat islets: Involvement of the cholinergic pathway | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1677/JOE-08-0309 | es |
sedici.identifier.other | eid:2-s2.0-56849110937 | es |
sedici.identifier.issn | 0022-0795 | es |
sedici.creator.person | Barbosa, Helena C. | es |
sedici.creator.person | Bordin, Silvana | es |
sedici.creator.person | Anhê, Gabriel | es |
sedici.creator.person | Persaud, Shanta J. | es |
sedici.creator.person | Bowe, James | es |
sedici.creator.person | Borelli, María Inés | es |
sedici.creator.person | Gagliardino, Juan José | es |
sedici.creator.person | Boschero, Antonio C. | es |
sedici.subject.materias | Ciencias Médicas | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Facultad de Ciencias Médicas | es |
mods.originInfo.place | Centro de Endocrinología Experimental y Aplicada | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Journal of Endocrinology | es |
sedici.relation.journalVolumeAndIssue | vol. 199, no. 2 | es |
sedici.rights.sherpa | * RoMEO: amarillo* Pre-print del autor: can* Post-print del autor: restricted* Versión de editor/PDF:cannot* Condiciones:>>On Institutional repository or Central repository>>La versión de editor/PDF no puede utilizarse>>La declaración establecida debe acompañar el depósito (ver Política)>>El pre-print no debe reemplazarse por el post-print, sino que se enlazará a la versión publicada con una declaración establecida corregida>>El editor depositará copia en PubMed Central en nombre de los autores financiados por el NIH>>Publisher last contacted on 06/06/2019* Link a Sherpa: http://sherpa.ac.uk/romeo/issn/0022-0795/es/ |