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dc.date.accessioned | 2019-10-28T17:54:32Z | |
dc.date.available | 2019-10-28T17:54:32Z | |
dc.date.issued | 2008 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/84246 | |
dc.description.abstract | A new cysteine peptidase (Granulosain I) was isolated from ripe fruits of Solanum granuloso-leprosum Dunal (Solanaceae) by means of precipitation with organic solvent and cation exchange chromatography. The enzyme showed a single band by SDS-PAGE, its molecular mass was 24,746 Da (MALDI-TOF/MS) and its isoelectric point was higher than 9.3. It showed maximum activity (more than 90%) in the pH range 7-8.6. Granulosain I was completely inhibited by E-64 and activated by the addition of cysteine or 2-mercaptoethanol, confirming its cysteinic nature. The kinetic studies carried out with PFLNA as substrate, showed an affinity (Km 0.6 mM) slightly lower than those of other known plant cysteine proteases (papain and bromelain). The N-terminal sequence of granulosain I (DRLPASVDWRGKGVLVLVKNQGQC) exhibited a close homology with other cysteine proteases belonging to the C1A family. | en |
dc.format.extent | 267-275 | es |
dc.language | en | es |
dc.subject | Cysteine protease | es |
dc.subject | Plant peptidases | es |
dc.subject | Solanaceae | es |
dc.subject | Solanum granuloso-leprosum | es |
dc.title | Granulosain I, a cysteine protease isolated from ripe fruits of Solanum granuloso-leprosum (Solanaceae) | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1007/s10930-008-9133-4 | es |
sedici.identifier.other | eid:2-s2.0-51449093504 | es |
sedici.identifier.issn | 1572-3887 | es |
sedici.creator.person | Vallés, Diego | es |
sedici.creator.person | Bruno, Mariela Anahí | es |
sedici.creator.person | López, Laura María Isabel | es |
sedici.creator.person | Caffini, Néstor Oscar | es |
sedici.creator.person | Cantera, Ana María B. | es |
sedici.subject.materias | Ciencias Exactas | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Facultad de Ciencias Exactas | es |
mods.originInfo.place | Centro de Investigación de Proteínas Vegetales | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Protein Journal | es |
sedici.relation.journalVolumeAndIssue | vol. 27, no. 5 | es |
sedici.rights.sherpa | * RoMEO: verde* Pre-print del autor: can* Post-print del autor: can* Versión de editor/PDF:cannot* Condiciones:>>Author's pre-print on pre-print servers such as arXiv.org>>Author's post-print on author's personal website immediately>>Author's post-print on any open access repository after 12 months after publication>>La versión de editor/PDF no puede utilizarse>>La fuente editorial debe reconocerse>>Debe ir enlazado a la versión de editor>>Set phrase to accompany link to published version (see policy)>>Los artículos de algunas revistas pueden hacerse de acceso abierto mediante el pago de una tarifa adicional>>Publisher last reviewed on 26/07/2016* Link a Sherpa: http://sherpa.ac.uk/romeo/issn/1572-3887/es/ |