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dc.date.accessioned 2019-10-28T17:54:32Z
dc.date.available 2019-10-28T17:54:32Z
dc.date.issued 2008
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/84246
dc.description.abstract A new cysteine peptidase (Granulosain I) was isolated from ripe fruits of Solanum granuloso-leprosum Dunal (Solanaceae) by means of precipitation with organic solvent and cation exchange chromatography. The enzyme showed a single band by SDS-PAGE, its molecular mass was 24,746 Da (MALDI-TOF/MS) and its isoelectric point was higher than 9.3. It showed maximum activity (more than 90%) in the pH range 7-8.6. Granulosain I was completely inhibited by E-64 and activated by the addition of cysteine or 2-mercaptoethanol, confirming its cysteinic nature. The kinetic studies carried out with PFLNA as substrate, showed an affinity (Km 0.6 mM) slightly lower than those of other known plant cysteine proteases (papain and bromelain). The N-terminal sequence of granulosain I (DRLPASVDWRGKGVLVLVKNQGQC) exhibited a close homology with other cysteine proteases belonging to the C1A family. en
dc.format.extent 267-275 es
dc.language en es
dc.subject Cysteine protease es
dc.subject Plant peptidases es
dc.subject Solanaceae es
dc.subject Solanum granuloso-leprosum es
dc.title Granulosain I, a cysteine protease isolated from ripe fruits of Solanum granuloso-leprosum (Solanaceae) en
dc.type Articulo es
sedici.identifier.other doi:10.1007/s10930-008-9133-4 es
sedici.identifier.other eid:2-s2.0-51449093504 es
sedici.identifier.issn 1572-3887 es
sedici.creator.person Vallés, Diego es
sedici.creator.person Bruno, Mariela Anahí es
sedici.creator.person López, Laura María Isabel es
sedici.creator.person Caffini, Néstor Oscar es
sedici.creator.person Cantera, Ana María B. es
sedici.subject.materias Ciencias Exactas es
sedici.description.fulltext true es
mods.originInfo.place Facultad de Ciencias Exactas es
mods.originInfo.place Centro de Investigación de Proteínas Vegetales es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Protein Journal es
sedici.relation.journalVolumeAndIssue vol. 27, no. 5 es
sedici.rights.sherpa * RoMEO: verde* Pre-print del autor: can* Post-print del autor: can* Versión de editor/PDF:cannot* Condiciones:>>Author's pre-print on pre-print servers such as arXiv.org>>Author's post-print on author's personal website immediately>>Author's post-print on any open access repository after 12 months after publication>>La versión de editor/PDF no puede utilizarse>>La fuente editorial debe reconocerse>>Debe ir enlazado a la versión de editor>>Set phrase to accompany link to published version (see policy)>>Los artículos de algunas revistas pueden hacerse de acceso abierto mediante el pago de una tarifa adicional>>Publisher last reviewed on 26/07/2016* Link a Sherpa: http://sherpa.ac.uk/romeo/issn/1572-3887/es/


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)