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dc.date.accessioned | 2019-10-30T12:49:05Z | |
dc.date.available | 2019-10-30T12:49:05Z | |
dc.date.issued | 2003 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/84343 | |
dc.description.abstract | Two DNA fragments, a 730-bp and a 900-bp fragment, one homologous to host cultivar specificity genes nolBT of Sinorhizobium fredii and the other one homologous to RSα, an insertion-like sequence present in Bradyrhizobium japonicum, were generated by polymerase chain reaction (PCR) with two pairs of primers. The amount of each fragment generated by the multiplex PCR was proportional to the amount of template DNA present. The amplification of the 900-bp RSα fragment was more sensitive, since it was amplified from a smaller amount of template DNA than the 730-bp nolBT fragment. By running the multiplex reaction in the presence of template DNA isolated from different sources, we confirmed that the reaction can discriminate between S. fredii, Bradyrhizobium japonicum and Sinorhizobium xinjiangensis. | en |
dc.format.extent | 153-158 | es |
dc.language | en | es |
dc.subject | Bradyrhizobium | es |
dc.subject | Molecular marker | es |
dc.subject | Polymerase chain reaction | es |
dc.subject | Rhizobium | es |
dc.subject | Sinorhizobium | es |
dc.subject | Soybean | es |
dc.title | Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1016/S0378-1097(03)00796-1 | es |
sedici.identifier.other | eid:2-s2.0-0346219329 | es |
sedici.identifier.issn | 0378-1097 | es |
sedici.creator.person | Pastorino, Graciela Noemí | es |
sedici.creator.person | Martínez Alcántara, Virginia | es |
sedici.creator.person | Balatti, Pedro Alberto | es |
sedici.subject.materias | Ciencias Agrarias | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Facultad de Ciencias Agrarias y Forestales | es |
mods.originInfo.place | Instituto de Fisiología Vegetal | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | FEMS Microbiology Letters | es |
sedici.relation.journalVolumeAndIssue | vol. 229, no. 2 | es |
sedici.rights.sherpa | * RoMEO: verde* Pre-print del autor: can* Post-print del autor: can* Versión de editor/PDF:cannot* Condiciones:>>El pre-print sólo puede depositarse antes de la aceptación>>El pre-print debe acompañarse de una declaración establecida (ver enlace)>>El pre-print no debe reemplazarse por el post-print, sino que se enlazará a la versión publicada con una declaración establecida corregida>>Pre-print on author's personal website, employer website, free public server or pre-prints in subject area>>Post-print en el sitio web personal del autor de manera inmediata>>Post-print in Institutional repositories or Central repositories after 12 months embargo>>La versión de editor/PDF no puede utilizarse>>La fuente editorial debe reconocerse>>Debe ir enlazado a la versión de editor>>La copia archivada debe acompañarse de la frase establecida (ver Política)>>El editor depositará copia en PubMed Central en nombre de los autores financiados por el NIH>>Publisher last contacted on 19/02/2015* Link a Sherpa: http://sherpa.ac.uk/romeo/issn/0378-1097/es/ |