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dc.date.accessioned 2019-10-30T18:59:17Z
dc.date.available 2019-10-30T18:59:17Z
dc.date.issued 2003
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/84453
dc.description.abstract Objective: This work was aimed to prove that release/formation of endogenous endothelin acting in an autocrine/paracrine fashion contributes to the increase in contractility promoted by a low dose of angiotensin II. Methods: Isolated cat papillary muscles were used for force, pHi, [Na+]i and [Ca2+]i measurements and isolated cat myocytes for patch-clamp experiments. Results: In papillary muscles, 1.0 nmol/l angiotensin II increased force by 23±2% (n=4, P<0.05), [Na+]i by 2.2±0.2 mmol/l (n=4, P<0.05), and peak (but not diastolic) Ca2+ from 0.674±0.11 to 0.768±0.13 μmol/l (n=4, P<0.05), without affecting pH i. Force and [Na+]i increase were abolished by inhibition of the Na+/H+ exchanger (NHE) with the inhibitor HOE642, blockade of endothelin receptors with the nonselective antagonist TAK044 and by inhibition of the endothelin-converting enzyme with phosphoramidon. Force but not [Na+]i increase was abolished by inhibition of reverse Na+/Ca2+ exchange (NCX) with the inhibitor KB-R7943. Similar increase in force (21±2%, n=4, P<0.05) and in [Na+]i (2.4±0.4 mmol/l, n=4, P<0.05) that were also suppressed by TAK044 and HOE642 were induced by exogenous 5.0 nmol/l endothelin-1. KB-R7943 reverted the endothelin-1 effect on force but not on [Na+]i. In isolated myocytes, exogenous endothelin-1 dose-dependently increased the NCX current and shifted the NCX reversal potential (ENCX) to a more negative value (ΔENCX: -10±3 and -17±5 mV, with 1 and 10 nmol/l endothelin-1, respectively, n=12). The latter effect was prevented by HOE642. Conclusion: Taken together, the results indicate that a low dose of angiotensin II induces release of endothelin, which, in autocrine/paracrine fashion activates the Na+/H+ exchanger, increases [Na+]i and changes ENCX, promoting the influx of Ca2+ that leads to a positive inotropic effect (PIE). en
dc.format.extent 589-597 es
dc.language en es
dc.subject Angiotensin es
dc.subject Cat papillary muscles es
dc.subject E-C coupling es
dc.subject Endothelins es
dc.subject Ion transport es
dc.subject Isolated cat myocytes es
dc.subject Na/Ca-exchanger es
dc.subject Na/H-exchanger es
dc.title A low dose of angiotensin II increases inotropism through activation of reverse Na+/Ca2+ exchange by endothelin release en
dc.type Articulo es
sedici.identifier.other doi:10.1016/j.cardiores.2003.09.004 es
sedici.identifier.other eid:2-s2.0-0344196795 es
sedici.identifier.issn 0008-6363 es
sedici.creator.person Pérez, Néstor Gustavo es
sedici.creator.person Villa Abrille, María Celeste es
sedici.creator.person Aiello, Ernesto Alejandro es
sedici.creator.person Dulce, Raúl Ariel es
sedici.creator.person Cingolani, Horacio Eugenio es
sedici.creator.person Camilión de Hurtado, María Cristina es
sedici.subject.materias Ciencias Médicas es
sedici.description.fulltext true es
mods.originInfo.place Facultad de Ciencias Médicas es
mods.originInfo.place Centro de Investigaciones Cardiovasculares es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Cardiovascular Research es
sedici.relation.journalVolumeAndIssue vol. 60, no. 3 es
sedici.rights.sherpa * RoMEO: verde* Pre-print del autor: can* Post-print del autor: can* Versión de editor/PDF:cannot* Condiciones:>>El pre-print sólo puede depositarse antes de la aceptación>>El pre-print debe acompañarse de una declaración establecida (ver enlace)>>El pre-print no debe reemplazarse por el post-print, sino que se enlazará a la versión publicada con una declaración establecida corregida>>Pre-print on author's personal website, employer website, free public server or pre-prints in subject area>>Post-print en el sitio web personal del autor de manera inmediata>>Post-print in Institutional repositories or Central repositories after 12 months embargo>>La versión de editor/PDF no puede utilizarse>>La fuente editorial debe reconocerse>>Debe ir enlazado a la versión de editor>>La copia archivada debe acompañarse de la frase establecida (ver Política)>>El editor depositará copia en PubMed Central en nombre de los autores financiados por el NIH>>Publisher last contacted on 19/02/2015* Link a Sherpa: http://sherpa.ac.uk/romeo/issn/0008-6363/es/


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Excepto donde se diga explícitamente, este item se publica bajo la siguiente licencia Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)