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dc.date.accessioned 2019-11-14T18:01:41Z
dc.date.available 2019-11-14T18:01:41Z
dc.date.issued 2014
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/85638
dc.description.abstract Aging is associated to disrupted contractility and rhythmicity, among other cardiovascular alterations. Drosophila melanogaster shows a pattern of aging similar to human beings and recapitulates the arrhythmogenic conditions found in the human heart. Moreover, the kinase CaMKII has been characterized as an important regulator of heart function and an arrhythmogenic molecule that participate in Ca2+ handling. Using a genetically engineered expressed Ca2+ indicator, we report changes in cardiac Ca 2+ handling at two different ages. Aging prolonged relaxation, reduced spontaneous heart rate (HR) and increased the occurrence of arrhythmias, ectopic beats and asystoles. Alignment between Drosophila melanogaster and human CaMKII showed a high degree of conservation and indicates that relevant phosphorylation sites in humans are also present in the fruit fly. Inhibition of CaMKII by KN-93 (CaMKII-specific inhibitor), reduced HR without significant changes in other parameters. By contrast, overexpression of CaMKII increased HR and reduced arrhythmias. Moreover, it increased fluorescence amplitude, maximal rate of rise of fluorescence and reduced time to peak fluorescence. These results suggest that CaMKII in Drosophila melanogaster acts directly on heart function and that increasing CaMKII expression levels could be beneficial to improve contractility. en
dc.language en es
dc.subject Drosophila melanogaster es
dc.title Aging and CaMKII alter intracellular Ca2+ transients and heart rhythm in Drosophila melanogaster en
dc.type Articulo es
sedici.identifier.other doi:10.1371/journal.pone.0101871 es
sedici.identifier.other eid:2-s2.0-84904015554 es
sedici.identifier.issn 1932-6203 es
sedici.creator.person Santalla, Manuela es
sedici.creator.person Valverde, Carlos Alfredo es
sedici.creator.person Harnichar, E. es
sedici.creator.person Lacunza, Ezequiel es
sedici.creator.person Aguilar Fuentes, J. es
sedici.creator.person Mattiazzi, Alicia Ramona es
sedici.creator.person Ferrero, Paola Viviana es
sedici.subject.materias Ciencias Médicas es
sedici.description.fulltext true es
mods.originInfo.place Centro de Investigaciones Cardiovasculares es
mods.originInfo.place Centro de Investigaciones Inmunológicas Básicas y Aplicadas es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle PLoS ONE es
sedici.relation.journalVolumeAndIssue vol. 9, no. 7 es
sedici.rights.sherpa * Color: green * Pre-print del autor: si * Post-print del autor: si * Versión de editor/PDF:si * Condiciones: >>Creative Commons Attribution License 4.0 >>Authors retain copyright >>Publisher's version/PDF may be used >>Published source must be acknowledged with citation >>Author's pre-prints si be deposited in pre-print servers >>Publisher will deposit articles in PubMed Central >>All titles are open access journals * Link a Sherpa: http://sherpa.ac.uk/romeo/issn/1932-6203/es/


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Excepto donde se diga explícitamente, este item se publica bajo la siguiente licencia Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)