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dc.date.accessioned | 2019-12-17T16:14:16Z | |
dc.date.available | 2019-12-17T16:14:16Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/87580 | |
dc.description.abstract | A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with Gluconobacter Thailandicus NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca2+. D-Pentonate 4-dehydrogenase was purified to two different subunits, 80 and 14 kDa. The absorption spectrum of the purified enzyme showed no typical absorbance over the visible regions. The enzyme oxidized D-pentonates to 4-keto-D-pentonates at the optimum pH of 4.0. In addition, the enzyme oxidized D-fructose to 5-keto-D-fructose, D-psicose to 5-keto-D-psicose, including the other polyols such as, glycerol, D-ribitol, D-arabitol, and D-sorbitol. Thus, D-pentonate 4-dehydrogenase was found to be identical with glycerol dehydrogenase (GLDH), a major polyol dehydrogenase in Gluconobacter species. The reaction versatility of quinoprotein GLDH was notified in this study. | en |
dc.format.extent | 411-418 | es |
dc.language | en | es |
dc.subject | 4-keto-D-arabonate production | es |
dc.subject | 4-keto-D-pentonate | es |
dc.subject | Acetic acid bacteria | es |
dc.subject | Glycerol dehydrogenase | es |
dc.subject | Oxidative fermentation | es |
dc.title | Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1080/09168451.2016.1254535 | es |
sedici.identifier.other | eid:2-s2.0-85009726766 | es |
sedici.identifier.issn | 0916-8451 | es |
sedici.creator.person | Ano, Yoshitaka | es |
sedici.creator.person | Hours, Roque Alberto | es |
sedici.creator.person | Akakabe, Yoshihiko | es |
sedici.creator.person | Kataoka, Naoya | es |
sedici.creator.person | Yakushi, Toshiharu | es |
sedici.creator.person | Matsushita, Kazunobu | es |
sedici.creator.person | Adachi, Osao | es |
sedici.subject.materias | Ciencias Exactas | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Facultad de Ciencias Exactas | es |
mods.originInfo.place | Centro de Investigación y Desarrollo en Fermentaciones Industriales | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Bioscience, Biotechnology and Biochemistry | es |
sedici.relation.journalVolumeAndIssue | vol. 81, no. 2 | es |
sedici.rights.sherpa | * Color: green
* Pre-print del autor: si
* Post-print del autor: si
* Versión de editor/PDF:no
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