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dc.date.accessioned 2020-06-04T16:28:56Z
dc.date.available 2020-06-04T16:28:56Z
dc.date.issued 2018-06
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/97514
dc.description.abstract Bradyrhizobium diazoefficiens, a soybean N2-fixing symbiont, constitutes the basic input in one of the most prominent inoculant industries worldwide. This bacterium may be cultured with D-mannitol or L-arabinose as carbon-plus-energy source (C-source) with similar specific growth rates, but with higher biomass production with D-mannitol. To better understand the bacterium's carbon metabolism, we analyzed, by liquid chromatography and tandem mass spectrometry (MS), the whole set of proteins obtained from cells grown on each C-source. Among 3,334 proteins identified, 266 were overproduced in D-mannitol and 237 in L-arabinose, but among these, only 22% from D-mannitol cultures and 35% from L-arabinose cultures were annotated with well defined functions. In the D-mannitol-differential pool we found 19 enzymes of the pentose-phosphate and Calvin-Benson-Bassham pathways and accordingly observed increased extracellular-polysaccharide production by D-mannitol grown bacteria in a CO2-enriched atmosphere. Moreover, poly-3-hydroxybutyrate biosynthesis was increased, suggesting a surplus of reducing power. In contrast, the L-arabinose-differential pool contained 11 enzymes of the L-2-keto-3-deoxyarabonate pathway, 4 enzymes for the synthesis of nicotinamide-adenine dinucleotide from aspartate, with those cultures having a threefold higher O2-consumption rate than the D-mannitol cultures. The stoichiometric balances deduced from the modeled pathways, however, resulted in similar O2 consumptions and ATP productions per C-mole of substrate. These results suggested higher maintenance-energy demands in L-arabinose, which energy may be used partly for flagella-driven motility. Since B. diazoefficiens produces the lateral-flagella system in only L-arabinose, we calculated the O2-consumption rates of a lafR::Km mutant devoid of lateral flagella cultured in L-arabinose or D-mannitol. Contrary to that of the wild-type, the O2-consumption rate of this mutant was similar on both C-sources, and accordingly outcompeted the wild-type in coculture, suggesting that the lateral flagella behaved as parasitic structures under these conditions. Proteomic data are available via ProteomeXchange with identifier PXD008263. en
dc.format.extent 1-16 es
dc.language en es
dc.subject Bradyrhizobium es
dc.subject CBB pathway es
dc.subject Flagella es
dc.subject L-KDA pathway es
dc.subject O2 consumption es
dc.subject PP pathway es
dc.title Induction by Bradyrhizobium diazoefficiens of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO2 Fixation, O2 Consumption, and Lateral-Flagellum Production en
dc.type Articulo es
sedici.identifier.uri https://ri.conicet.gov.ar/11336/96974 es
sedici.identifier.uri https://www.frontiersin.org/articles/10.3389/fmicb.2018.01189/full es
sedici.identifier.uri https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996035/ es
sedici.identifier.other http://dx.doi.org/10.3389/fmicb.2018.01189 es
sedici.identifier.other hdl:11336/96974 es
sedici.identifier.issn 1664-302X es
sedici.creator.person Cogo, Carolina es
sedici.creator.person Pérez Giménez, Julieta es
sedici.creator.person Rajeswari, Chandrasekar B. es
sedici.creator.person Luna, María Flavia es
sedici.creator.person Lodeiro, Aníbal Roberto es
sedici.subject.materias Biología es
sedici.description.fulltext true es
mods.originInfo.place Instituto de Biotecnologia y Biologia Molecular es
mods.originInfo.place Centro de Investigación y Desarrollo en Fermentaciones Industriales es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Frontiers in Microbiology es
sedici.relation.journalVolumeAndIssue vol. 9 es


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Excepto donde se diga explícitamente, este item se publica bajo la siguiente licencia Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)