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dc.date.accessioned | 2020-06-04T16:28:56Z | |
dc.date.available | 2020-06-04T16:28:56Z | |
dc.date.issued | 2018-06 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/97514 | |
dc.description.abstract | Bradyrhizobium diazoefficiens, a soybean N2-fixing symbiont, constitutes the basic input in one of the most prominent inoculant industries worldwide. This bacterium may be cultured with D-mannitol or L-arabinose as carbon-plus-energy source (C-source) with similar specific growth rates, but with higher biomass production with D-mannitol. To better understand the bacterium's carbon metabolism, we analyzed, by liquid chromatography and tandem mass spectrometry (MS), the whole set of proteins obtained from cells grown on each C-source. Among 3,334 proteins identified, 266 were overproduced in D-mannitol and 237 in L-arabinose, but among these, only 22% from D-mannitol cultures and 35% from L-arabinose cultures were annotated with well defined functions. In the D-mannitol-differential pool we found 19 enzymes of the pentose-phosphate and Calvin-Benson-Bassham pathways and accordingly observed increased extracellular-polysaccharide production by D-mannitol grown bacteria in a CO2-enriched atmosphere. Moreover, poly-3-hydroxybutyrate biosynthesis was increased, suggesting a surplus of reducing power. In contrast, the L-arabinose-differential pool contained 11 enzymes of the L-2-keto-3-deoxyarabonate pathway, 4 enzymes for the synthesis of nicotinamide-adenine dinucleotide from aspartate, with those cultures having a threefold higher O2-consumption rate than the D-mannitol cultures. The stoichiometric balances deduced from the modeled pathways, however, resulted in similar O2 consumptions and ATP productions per C-mole of substrate. These results suggested higher maintenance-energy demands in L-arabinose, which energy may be used partly for flagella-driven motility. Since B. diazoefficiens produces the lateral-flagella system in only L-arabinose, we calculated the O2-consumption rates of a lafR::Km mutant devoid of lateral flagella cultured in L-arabinose or D-mannitol. Contrary to that of the wild-type, the O2-consumption rate of this mutant was similar on both C-sources, and accordingly outcompeted the wild-type in coculture, suggesting that the lateral flagella behaved as parasitic structures under these conditions. Proteomic data are available via ProteomeXchange with identifier PXD008263. | en |
dc.format.extent | 1-16 | es |
dc.language | en | es |
dc.subject | Bradyrhizobium | es |
dc.subject | CBB pathway | es |
dc.subject | Flagella | es |
dc.subject | L-KDA pathway | es |
dc.subject | O2 consumption | es |
dc.subject | PP pathway | es |
dc.title | Induction by Bradyrhizobium diazoefficiens of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO2 Fixation, O2 Consumption, and Lateral-Flagellum Production | en |
dc.type | Articulo | es |
sedici.identifier.uri | https://ri.conicet.gov.ar/11336/96974 | es |
sedici.identifier.uri | https://www.frontiersin.org/articles/10.3389/fmicb.2018.01189/full | es |
sedici.identifier.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996035/ | es |
sedici.identifier.other | http://dx.doi.org/10.3389/fmicb.2018.01189 | es |
sedici.identifier.other | hdl:11336/96974 | es |
sedici.identifier.issn | 1664-302X | es |
sedici.creator.person | Cogo, Carolina | es |
sedici.creator.person | Pérez Giménez, Julieta | es |
sedici.creator.person | Rajeswari, Chandrasekar B. | es |
sedici.creator.person | Luna, María Flavia | es |
sedici.creator.person | Lodeiro, Aníbal Roberto | es |
sedici.subject.materias | Biología | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Instituto de Biotecnologia y Biologia Molecular | es |
mods.originInfo.place | Centro de Investigación y Desarrollo en Fermentaciones Industriales | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Frontiers in Microbiology | es |
sedici.relation.journalVolumeAndIssue | vol. 9 | es |