We present a checklist of the melittofauna from La Tatacoa Desert, Department of Huila, a remnant of highly disturbed tropical dry forest located in the upper valley of the Magdalena River. The list consists of 33 species in 20 genera of three families. The most abundant species was the emphorine bee Melitomella schwarzi (Michener, 1954), which accounted for 43% of the specimens collected. Exomalopsis (Phanomalopsis) trifasciata Brèthes, 1910 and Paratetrapedia connexa (Vachal, 1909) are newly recorded for Colombia.