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dc.date.accessioned 2020-09-07T16:51:12Z
dc.date.available 2020-09-07T16:51:12Z
dc.date.issued 2017
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/104004
dc.description.abstract UV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log10 reductions (1.5 J/cm2) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log10 reductions (3.0 J/cm2) showed mutagenicity with metabolic activation. en
dc.format.extent 315-330 es
dc.language en es
dc.subject Mutagenicity es
dc.subject UV radiation es
dc.subject Orange juice es
dc.subject Ames test es
dc.subject Ascorbic acid es
dc.title Evaluation of mutagenicity associated with Escherichia coli inactivation in UV-treated orange juice en
dc.type Articulo es
sedici.identifier.other https://doi.org/10.1080/02772248.2016.1178752 es
sedici.identifier.issn 1029-0486 es
sedici.creator.person Rodríguez, María es
sedici.creator.person Oteiza, Juan Martín es
sedici.creator.person Giannuzzi, Leda es
sedici.creator.person Zaritzky, Noemí Elisabet es
sedici.subject.materias Química es
sedici.description.fulltext true es
mods.originInfo.place Centro de Investigación y Desarrollo en Criotecnología de Alimentos es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Toxicological & Environmental Chemistry es
sedici.relation.journalVolumeAndIssue vol. 99, no. 2 es


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Excepto donde se diga explícitamente, este item se publica bajo la siguiente licencia Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)