In this work we analyzed the karyotype of Rhinella jimi (Stevaux, 2002) (Anura, Bufonidae) from Picos (Piauí) in Northeastern Brazil. The chromosomes were examined using classical cytogenetic approaches (Giemsa, C-banding, and Ag-NOR staining). This species has 2n = 22 chromosomes, all metacentric or submetacentric. Heterochromatic segments were visualized at the centromeric region and the nucleolus organizer regions (NOR) were restricted to terminal regions of the short arms in pair 7. There was no evidence of heteromorphic sex chromosomes.
The chromosomal analysis of R. jimi allowed us to identify a karyotype that is similar to many other species of Rhinella, in which the diploid number remains unchanged and without evidences of structural rearrangements.