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dc.date.accessioned 2020-10-27T18:55:30Z
dc.date.available 2020-10-27T18:55:30Z
dc.date.issued 1991
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/107831
dc.description.abstract Tn5-induced mutants of Rhizobium meliloti that require the amino acids isoleucine and valine for growth on minimal medium were studied. In one mutant, 1028, the defect is associated with an inability to induce nodules on alfalfa. The Tn5 mutation in 1028 is located in a chromosomal 5.5-kb EcoRI fragment.- Complementation analysis with cloned DNA indicated that 2.0 kb of DNA from the 5.5-kb EcoRI fragment restored the wild-type phenotype in the Ilv⁻ Nod⁻ mutant. This region was further characterized by DNA sequence analysis and was shown to contain a coding sequence homologous to those for Escherichia coli DJvC and Saccharomyces cerevisiae Ilv5. Genes ilvC and ilv5 code for the enzyme acetohydroxy acid isomeroreductase (isomeroreductase), the second enzyme in the parallel pathways for the biosynthesis of isoleucine and valine. Enzymatic assays confirmed that strain 1028 was a mutant defective in isomeroreductase activity. In addition, it was shown that the ilvC genes of Rhizobium meliloti and E. coli are functionally equivalent. We demonstrated that in ilvC mutant 1028 the common nodulation genes nodABC are not activated by the inducer luteolin. E. coli ilvC complemented both defective properties (Ilv⁻ and Nod⁻) found in mutant 1028. These findings demonstrate that R. meliloti requires an active isomeroreductase enzyme for successful nodulation of alfalfa. en
dc.format.extent 7756-7764 es
dc.language en es
dc.subject Rhizobium meliloti es
dc.subject Alfalfa es
dc.title The product of the Rhizobium meliloti ilvC gene is required for isoleucine and valine synthesis and nodulation of alfalfa en
dc.type Articulo es
sedici.identifier.uri http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC212565&blobtype=pdf es
sedici.identifier.uri https://jb.asm.org/content/173/24/7756 es
sedici.identifier.other pmid:1744032 es
sedici.identifier.other pmcid:PMC212565 es
sedici.identifier.other http://dx.doi.org/10.1128/jb.173.24.7756-7764.1991 es
sedici.identifier.issn 0021-9193 es
sedici.creator.person Aguilar, Orlando Mario es
sedici.creator.person Grasso, Daniel Horacio es
sedici.subject.materias Ciencias Exactas es
sedici.description.fulltext true es
mods.originInfo.place Facultad de Ciencias Exactas es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Journal of Bacteriology es
sedici.relation.journalVolumeAndIssue vol. 173, no. 24 es


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)