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dc.date.accessioned | 2021-08-31T17:27:51Z | |
dc.date.available | 2021-08-31T17:27:51Z | |
dc.date.issued | 2005 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/123858 | |
dc.description.abstract | The immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different. | en |
dc.format.extent | 17-28 | es |
dc.language | en | es |
dc.subject | β-conglycinin | es |
dc.subject | Recombinant soybean subunits | es |
dc.subject | Immunoreactivity | es |
dc.subject | Soybean 7S globulin | es |
dc.title | Immunochemical reactivity of soybean β-conglycinin subunits | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1080/09540100500054727 | es |
sedici.identifier.issn | 0954-0105 | es |
sedici.identifier.issn | 1465-3443 | es |
sedici.creator.person | Petruccelli, Silvana | es |
sedici.creator.person | Chirdo, Fernando Gabriel | es |
sedici.creator.person | Añón, María Cristina | es |
sedici.subject.materias | Química | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Centro de Investigación y Desarrollo en Criotecnología de Alimentos | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Food and Agricultural Immunology | es |
sedici.relation.journalVolumeAndIssue | vol. 16, no. 1 | es |