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dc.date.accessioned | 2022-03-25T18:46:44Z | |
dc.date.available | 2022-03-25T18:46:44Z | |
dc.date.issued | 1999-08 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/133325 | |
dc.description.abstract | A modification of the ecometric method was developed for a rapid screening of bile resistance in lactic acid bacteria and bifidobacteria. Validation of the MEM bile assay (modified ecometric method) was performed comparing the bile resistance index (RIbile) and the bile resistance ratio (R%). Most Bifidobacterium strains assayed were bile sensitive (83.3%), while only 62.1% of lactobacilli showed that behavior. Some bifidobacterial strains (55.6%) showed a crystalline precipitate when grown on solid medium supplemented with 0.5% ox bile. The crystalline structures produced by B. pseudolongum CIDCA 531 were isolated and analyzed by optical and scanning electron microscopy, thin-layer chromatography, melting point, and specific cholesterol reactions. Those studies confirmed the presence of cholesterol in these crystalline structures. On the other hand, none of the lactobacilli and streptococci studied had the ability to produce crystalline precipitates. | en |
dc.format.extent | 905-912 | es |
dc.language | en | es |
dc.subject | bile resistance | es |
dc.subject | lactic acid bacteria | es |
dc.subject | bifidobacteria | es |
dc.subject | ecometric method | es |
dc.title | Screening of Bile Resistance and Bile Precipitation in Lactic Acid Bacteria and Bifidobacteria | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.4315/0362-028x-62.8.905 | es |
sedici.identifier.other | pmid:10456745 | es |
sedici.identifier.issn | 0362-028x | es |
sedici.identifier.issn | 1944-9097 | es |
sedici.creator.person | Kociubinski, Guillermo Luis | es |
sedici.creator.person | Pérez, Pablo Fernando | es |
sedici.creator.person | De Antoni, Graciela Liliana | es |
sedici.subject.materias | Ciencias Exactas | es |
sedici.subject.materias | Biología | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Facultad de Ciencias Exactas | es |
mods.originInfo.place | Centro de Investigación y Desarrollo en Criotecnología de Alimentos | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Journal of Food Protection | es |
sedici.relation.journalVolumeAndIssue | vol. 62, no. 8 | es |