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dc.date.accessioned | 2022-07-04T13:49:41Z | |
dc.date.available | 2022-07-04T13:49:41Z | |
dc.date.issued | 1999-06 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/138784 | |
dc.description.abstract | Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of protopectin used. As PPase-SE also shows polygalacturonase (PGase) activity, a method for the assay of this activity was optimised. The best assay conditions were: substrate (polygalacturonic acid) concentration of 2.0 g 1−1, reaction time of 10 min and up to 0.17 PGase units per test tube. | en |
dc.format.extent | 385-390 | es |
dc.language | en | es |
dc.subject | endopolygalacturonases | es |
dc.subject | Geotrichum klebahnii | es |
dc.subject | pectin | es |
dc.subject | pectin-releasing enzymes | es |
dc.subject | protopectinases | es |
dc.title | Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1023/a:1008917912296 | es |
sedici.identifier.issn | 0951-208x | es |
sedici.identifier.issn | 1573-6784 | es |
sedici.creator.person | Cavalitto, Sebastián Fernando | es |
sedici.creator.person | Hours, Roque Alberto | es |
sedici.creator.person | Mignone, Carlos Fernando | es |
sedici.subject.materias | Ciencias Exactas | es |
sedici.subject.materias | Física | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Centro de Investigación y Desarrollo en Fermentaciones Industriales | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Biotechnology Techniques | es |
sedici.relation.journalVolumeAndIssue | vol. 13, no. 6 | es |