Twelve species of anurans (Amazophrynella teko, Rhinella castaneotica, Hyalinobatrachium mondolfii, H. tricolor, Pristimantis gutturalis, P. inguinalis, Ranitomeya variabilis, Osteocephalus leprieurii, Scinax proboscideus, Leptodactylus petersii, Chiasmocleis hudsoni, Synapturanus mirandaribeiroi), into the seven families (Bufonidae, Centrolenidae, Craugastoridae, Dendrobatidae, Hylidae, Leptodactylidae, Microhylidae) are reported for the first time from Cancão Municipal Natural Park, state of Amapá, North Brazil. The total number of species of anurans known from the park now stands at 61 species. Our results contribute to an increase in the knowledge of the anuran fauna of Eastern Amazonia and Guiana Shield.