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Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina

Belongs to book: Regional network for Latin America on animal disease diagnosis using immunoassay and labelled DNA probe techniques
Document type: Objeto de conferencia

Abstract

Indirect and dot enzyme linked immunosorbent assays (ELISAs) were developed to detect antibodies to pseudorabies virus (PRV) in serum samples of 321 pigs. The Argentine CL 15 PRV strain was used as the source of virus. Nonidet P-40-solubilized infected and mock infected cell lysates were used as antigens. The results of these tests were compared with those of a blocking ELISA and virus neutralization (VN) test. There was a 100% agreement between the indirect and dot-ELISAs. Using VN as standard, the blocking ELISA and the indirect ELISA showed 97.6% and 100% relative sensitivity and 96.9% and 99% relative specificity respectively. The sensitivity of the blocking ELISA was lower than that of the other tests.

General information

Exposure date: 1990
Issue date: julio 1992
Document language: English
Event: FAO/IAEA/SIDA Meeting (Heredia, Costa Rica, 22-26 October, 1990)
Origin: Facultad de Ciencias Veterinarias
Pages: 59-64
xmlui.dri2xhtml.METS-1.0.item-dc-subject: ELISA ; Técnicas de laboratorio ; Porcinos
Subjects: Ciencias Veterinarias

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Created: 11 de abril de 2023
Available since: 11 de abril de 2023
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  • http://sedici.unlp.edu.ar/handle/10915/151289

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