A novel series of 5-benzylidene-2-[(pyridine-2-ylmethylene)hydrazono]-thiazolidin-4-ones 3a-i has
been synthesized. 2-[(Pyridine-2-ylmethylene)hydrazono]-thiazolidin-4-ones 2a-c were also obtained and used as
intermediates to give the target compounds. The in vitro cytotoxic activity was evaluated for both series. The
findings obtained showed that the compounds 2a, 2b, 3b and 3c were effective against the HEp-2 cell lines with
IC50
in the 1.6 - 0.5 μg/mL range, whereas the compounds 2a (IC50
= 3.6 μg/mL), 2b (IC50
= 2.4 μg/mL) and 3f
(IC50
= 3.5 μg/mL) showed good inhibitory effects against HT-29 cell lines. As complementary biological test,
all 4-thiazolidinones were evaluated for antimicrobial activity against various bacterial and fungal species.