We collected 28 adult anurans belonging to six species (five Dendropsophus nanus, ten Hypsiboas pulchellus, one Leptodactylus latrans, ten Rhinella fernandezae, one R. schneideri, and one Scinax nasicus). To collect DNA samples for looking Bd infection through molecular techniques, all specimens were gently but firmly swabbed several times on the ventral surface, hindlimbs and interdigital membrane following the techniques of Livo (2004) and Hyatt et al. (2007).