The arboreal, nocturnal and widely distributed snake Bothrops bilineatus smaragdinus (Squamata, Viperidae) is known as a generalist whose diet items have rarely been identified to species level. In this work we report three new items for its diet: an adult frog Dendropsophus marmoratus (Anura, Hylidae), an adult lizard Thecadactylus solimoensis (Squamata, Phyllodactylidae), and an adult bat Carollia brevicauda (Chiroptera, Phyllostomidae), the latter being the first record of predation of a Chiropteran by this species.