HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)

Upadhyaya, Sunil Datt; Nayak, Preeti Sagar; Upadhyaya, Anubha

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HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)

Autores: Upadhyaya, Sunil Datt | Nayak, Preeti Sagar | Upadhyaya, Anubha

2010

Tipo de documento: Comunicacion

Resumen

A simple procedure was described for the determination of Withanolide A and Withanolide B in Ashwagandha root by HPLC. Separation was achieved on a Spherisorb C18 column with Water: Acetonitrile in the gradient elution. Detection was accomplished with UV detection at λ = 216 nm. Using this method, the bioactive steroid in Withania somnifera was determined. Significant variations in the contents of Withanolide A and Withanolide B in Ashwagandha root growing in different locations were also observed.