The use of biosensors based on laccase is an interesting alternative for monitoring phenolic compounds in pharmaceutical and environmental analysis. The detection and determination of acetaminophen in pharmaceutical samples, using a biosensor with carbon paste modified with laccase is described. The method showed good linearity
(r = 0.9954) and low detection and quantification limits (2.4 x 10-6 mol.L-1 and 7.9 x 10-6 mol.L-1, respectively). Furthermore, the proposed methodology using an enzyme electrode showed appropriated reproducibility and selectivity.