Comparative study on the pharmacokinetic of lansoprazole in gastric ulcer and normal rabbits by HPLC-DAD

Abstract

Gastric ulcer is one of ulcerous diseases and may result in some changes of many enzymes and transporters concerned with metabolism and disposal of drug. The pharmacokinetic of drug should be different between peptic ulcer and normal animals. Lansoprazole has been one of important medicine for treatment of ulcerous diseases. So, this paper investigated the difference of pharmacokinetic profiles of lansoprazole in gastric ulcer and normal rabbits in vivo by HPLC-DAD method. In this work, a liquid-liquid extraction and enrichment method with RP-HPLC determination route was taken. The pharmacokinetic parameters were analyzed by double-compartmental method (DAS2.0). The pharmacokinetic parameters of lansoprazole in normal and ulcer rabbits were as follows: (614.42 ± 152.25) and (875.73 ± 316.34) mg h/L for AUC(_0-6.5); (0.68 ± 0.12) and (0.83 ± 0.22) h for MRT(_0-6.5), (0.52 ± 0.23) and (0.87 ± 0.42) h for t_1/2 ; (6.13 ± 2.11) and (2.54 ± 1.65) L/h/kg for CL, respectively

Gastric ulcer is one of ulcerous diseases and may result in some changes of many enzymes and transporters concerned with metabolism and disposal of drug. The pharmacokinetic of drug should be different between peptic ulcer and normal animals. Lansoprazole has been one of important medicine for treatment of ulcerous diseases. So, this paper investigated the difference of pharmacokinetic profiles of lansoprazole in gastric ulcer and normal rabbits in vivo by HPLC-DAD method. In this work, a liquid-liquid extraction and enrichment method with RP-HPLC determination route was taken. The pharmacokinetic parameters were analyzed by double-compartmental method (DAS2.0). The pharmacokinetic parameters of lansoprazole in normal and ulcer rabbits were as follows: (614.42 ± 152.25) and (875.73 ± 316.34) mg h/L for AUC(_0-6.5); (0.68 ± 0.12) and (0.83 ± 0.22) h for MRT(_0-6.5 , (0.52 ± 0.23) and (0.87 ± 0.42) h for t_1/2 ; (6.13 ± 2.11) and (2.54 ± 1.65) L/h/kg for CL, respectively