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dc.date.accessioned 2019-10-17T13:19:05Z
dc.date.available 2019-10-17T13:19:05Z
dc.date.issued 2006-06-01
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/83449
dc.description.abstract We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes. en
dc.format.extent 4476-4485 es
dc.language en es
dc.subject Cyclooxygenase-2 es
dc.subject Prostaglandin F2 es
dc.subject Leydig cells es
dc.title Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells en
dc.type Articulo es
sedici.identifier.other http://dx.doi.org/10.1210/en.2006-0090 es
sedici.identifier.issn 0013-7227 es
sedici.title.subtitle Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production en
sedici.creator.person Frungieri, Mónica B. es
sedici.creator.person Gonzalez Calvar, Silvia I. es
sedici.creator.person Parborell, Fernanda es
sedici.creator.person Albrecht, Martin es
sedici.creator.person Mayerhofer, Artur es
sedici.creator.person Calandra, Ricardo Saúl es
sedici.subject.materias Bioquímica es
sedici.subject.materias Biología es
sedici.description.fulltext true es
mods.originInfo.place Instituto Multidisciplinario de Biología Celular es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Endocrinology es
sedici.relation.journalVolumeAndIssue vol. 147, no. 9 es


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Excepto donde se diga explícitamente, este item se publica bajo la siguiente licencia Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)