We describe and figure two distinctive new species of Neotropical carpenter bees of the genus Xylocopa, X. (Schonnherria) barbarae sp. n. from Bolivia, and X. (Schonnherria) bigibbosa sp. n. from Peru. To stabilize the names, we designate a neotype for X. (Schonnherria) dimidiata Latreille and lectotypes for the following species: X. (Neoxylocopa) andica Enderlein, X. (Neoxylocopa) columbiensis Pérez, X. (Neoxylocopa) mendozana Enderlein, and X. (Neoxylocopa) rufidorsum Enderlein. For each species, we provide comparative diagnosis, taxonomic comments, and summarize its distribution.