Analysis of geographic variation shows that populations of Bufo spinulosus Wiegmann are differentiated in Chile. Four recognizable, though broadly intergrading, population groups are indicated, corresponding to: (1) the northern mountainous arid environment of the Pune de Atacama plateau; (2) the isolated valleys and streams of the desert area of Atacama; (3) the central area between Coquimbo and Angol; and (4) the southern, humid Valdivian Forest.
Polymorphism was observed in some characters and clinal tendencies in others.