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dc.date.accessioned 2020-06-09T16:54:40Z
dc.date.available 2020-06-09T16:54:40Z
dc.date.issued 2012-08
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/97874
dc.description.abstract After depletion of intracellular Ca2+ stores the capacitative response triggers an extracellular Ca2+ influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca2+ depletion by a Ca2+-free extracellular solution followed by Ca2+ readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. In presence of 2-APB the magnitude of a second paired contraction by serotonin or histamine was significantly less than a first one, likely because 2-APB inhibited store refilling by capacitative Ca2+ entry. 2-APB inhibition of sarcoplasmic reticulum Ca2+ release was excluded because this blocker did not affect serotonin force development in a Ca2+-free solution. The PCR technique showed the presence of mRNAs for STIM proteins (1 and 2), for Orai proteins (1, 2 and 3) and for TRPC channels (subtypes 1, 3, 4 and 6) in the smooth muscle of the human umbilical artery. Hence, this artery presents a capacitative contractile response triggered by stimulation with physiological vasoconstrictors and expresses mRNAs for proteins and channels previously identified as SOCs. en
dc.format.extent 73-81 es
dc.language en es
dc.subject Human umbilical artery es
dc.subject Capacitative response es
dc.subject Trpc channels es
dc.subject Serotonin es
dc.subject Histamine es
dc.title Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels en
dc.type Articulo es
sedici.identifier.uri https://ri.conicet.gov.ar/11336/9616 es
sedici.identifier.uri http://www.ncbi.nlm.nih.gov/pubmed/23185782 es
sedici.identifier.uri http://www.cricyt.edu.ar/biocell/vol/pdf/36_2/04-Roldan.pdf es
sedici.identifier.other hdl:11336/9616 es
sedici.identifier.issn 0327-9545 es
sedici.creator.person Roldán Palomo, Ana Rocío es
sedici.creator.person Martín, Pedro es
sedici.creator.person Rebolledo, Alejandro es
sedici.creator.person Enrique, Nicolás Jorge es
sedici.creator.person Flores, Luis Emilio es
sedici.creator.person Milesi, María Verónica es
sedici.subject.materias Ciencias Exactas es
sedici.subject.materias Medicina es
sedici.description.fulltext true es
mods.originInfo.place Facultad de Ciencias Exactas es
mods.originInfo.place Facultad de Ciencias Médicas es
mods.originInfo.place Centro de Endocrinología Experimental y Aplicada es
mods.originInfo.place Grupo de Investigación en Fisiología Vascular es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Biocell es
sedici.relation.journalVolumeAndIssue vol. 36, no. 2 es


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