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dc.date.accessioned 2020-06-11T18:27:44Z
dc.date.available 2020-06-11T18:27:44Z
dc.date.issued 2017-12
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/98111
dc.description.abstract In this study we assessed the interaction of different strains of Bacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains of B. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquisition of lysosomal markers by B. cereus containing vacuoles (BcCV), assessed by LAMP1 and Lysotracker labelling occurred shortly after internalization. The highest ratio of LAMP1(+)-BcCV was found for strain M2. This strain was able to survive longer than strain B10502 which routes to LAMP1 containing vacuoles to a lesser extent. In addition, strain M2 stimulated expression of MHCII by infected cells. Confocal analyses 60 or 90 min post-infection showed different percentages of co-localization of bacteria with Lysotracker. Results suggest strain-dependent interaction and intracellular killing of B. cereus by phagocytic cells. These findings could be relevant for the pathogenic potential of Bacillus cereus strains. en
dc.format.extent 170-177 es
dc.language en es
dc.subject Bacillus cereus es
dc.subject Endocytic pathway es
dc.subject Intracellular trafficking es
dc.subject LAMP1 es
dc.subject Phagocytosis es
dc.subject Virulence es
dc.title Fate of Bacillus cereus within phagocytic cells en
dc.type Articulo es
sedici.identifier.uri https://ri.conicet.gov.ar/11336/57838 es
sedici.identifier.uri http://revistes.iec.cat/index.php/IM/article/view/144200 es
sedici.identifier.other http://dx.doi.org/10.2436/20.1501.01.300 es
sedici.identifier.other hdl:11336/57838 es
sedici.identifier.issn 1618-1905 es
sedici.creator.person Rolny, Ivanna Sabrina es
sedici.creator.person Racedo, Silvia María es
sedici.creator.person Pérez, Pablo Fernando es
sedici.subject.materias Biología es
sedici.description.fulltext true es
mods.originInfo.place Centro de Investigación y Desarrollo en Criotecnología de Alimentos es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle International Microbiology es
sedici.relation.journalVolumeAndIssue vol. 20, no. 4 es


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)