Subir material

Suba sus trabajos a SEDICI, para mejorar notoriamente su visibilidad e impacto

 

Mostrar el registro sencillo del ítem

dc.date.accessioned 2020-11-09T17:16:56Z
dc.date.available 2020-11-09T17:16:56Z
dc.date.issued 2005
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/108597
dc.description.abstract Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 μg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 μg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 μg and 14 μg respectively. en
dc.format.extent 170-176 es
dc.language en es
dc.subject Catalytic antibodies es
dc.subject In vitro cultures es
dc.subject Molecular farming es
dc.subject Recombinant antibodies es
dc.subject Tobacco es
dc.title Expression of the antibody 14D9 in Nicotiana tabacum hairy roots en
dc.type Articulo es
sedici.identifier.uri http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n2-10 es
sedici.identifier.issn 0717-3458 es
sedici.creator.person Martínez, Carolina es
sedici.creator.person Petruccelli, Silvana es
sedici.creator.person Giulietti, Ana María es
sedici.creator.person Alvarez, María Alejandra es
sedici.subject.materias Bioquímica es
sedici.description.fulltext true es
mods.originInfo.place Centro de Investigación y Desarrollo en Criotecnología de Alimentos es
mods.originInfo.place Universidad de Buenos Aires es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Electronic Journal of Biotechnology es
sedici.relation.journalVolumeAndIssue vol. 8, no. 2 es


Descargar archivos

Thumbnail
Documento completo
Descargar archivo (427.3Kb) - PDF
Icon
Enlace externo

Este ítem aparece en la(s) siguiente(s) colección(ones)

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Excepto donde se diga explícitamente, este item se publica bajo la siguiente licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)