Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots

Abstract

Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 μg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 μg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 μg and 14 μg respectively.