Cattle persistently infected (PI) with non-cytopathic (ncp) strains of bovine viral diarrhea virus (BVDV) are immunotolerant and have a major role in virus transmission. Also, they are highly prone to develop mucosal disease (MD) after an overwhelming infection with the mutant cytopathic (cp) strain. In August 2018, an outbreak investigation was conducted in a beef farm. Yearling steers and heifers died in a time-spanned fashion (30/205; mortality, 14.6 %). At necropsy two steers showed erosions and ulcers in the digestive organs and atrophy and necrosis of Peyer’s patches. BVDV antigen was detected in ear notch samples by immunochromatography, whereas the NS5B gene of BVDV-1 was detected in spleen samples by nested multiplex RT-PCR (RT-mPCR). Cytopathic effect of BVDV was confirmed by virus isolation and direct immunofluorescence (VI+DIF) in samples from brain, spleen, and lung.