Dengue fever is an important zoonotic disease in inter-tropical areas worldwide. In the past decades, the dengue virus (DEN) has spread into sub-tropical and temperate regions, with greater incidence and increasingly frequent epidemic outbreaks. In Argentina, DEN is an epidemic disease initiated by imported cases from countries where viral circulation is permanent. Santa Fe is a province in central Argentina where dengue outbreaks have been recorded since 2009, and the city of Reconquista has been the most affected since then.